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In vitro scientific studies, 25C-NBOMe has been shown to show cytotoxicity on neuronal cell strains SH-SY5Y, PC12, and SN471, as well as the compound was far more potent than methamphetamine at minimizing the visibility with the respective cells; the neurotoxicity of the compound consists of activation of MAPK/ERK cascade and inhibition of Akt/PKB